DESCRIPTION

CXCL2, also known as macrophage inflammatory protein 2-alpha (Mip2-alpha), is a member of CXC chemokine family that binds to CXC chemokine receptor 2 (CXCL2). CXCL2 is mainly produced by macrophages, endothelial cells, epithelial cells and tumor cells. CXCL2 plays important roles in various biological processes such as angiogenesis, inflammation, immune response and cancer biological behaviors. Under inflammatory conditions in CNS, microglia and astrocytes may secrete CXCL2 as well as other chemokines, which induce chemotaxis and infiltration of circulatory neutrophils. Indeed, previous studies suggest that CXCL2 is involved in Alzheimer's disease, multiple sclerosis and brain ischemia.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,039.9	14.7	1.4
2	8	254.8	4.4	1.7
3	8	131.3	2.3	1.8

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,035.0	18.7	1.8
2	16	259.4	6.8	2.6
3	16	130.8	2.1	1.6

RECOVERY

The recovery of mouse CXCL2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:2	86	81-94
Cell culture supernatant	1:800	109	105-114

LINEARITY

To assess the linearity of the assay, mouse serum samples were spiked with high concentrations of mouse CXCL2 and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Cell culture supernatant samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell culture supernatant was initially diluted 1:800.)

		Mouse serum	Cell culture supernatant
1:2	Average% of Expected	77	100
	Range (%)	75-78	-
1:4	Average% of Expected	90	103
	Range (%)	88-92	101-105
1:8	Average% of Expected	104	105
	Range (%)	103-105	102-107
1:16	Average% of Expected	109	102
	Range (%)	100-117	97-107

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CXCL2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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• Price: USD, per kit (48T / 96T options available)