DESCRIPTION

CD147 is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily. It plays a crucial role in various physiological and pathological processes, including immune responses, inflammation, and cancer progression. CD147 is a potent inducer of extracellular matrix metalloproteinases (MMPs), which are involved in extracellular matrix remodeling during processes such as wound healing, fibrosis, and cancer metastasis. Additionally, CD147 interacts with multiple partners, including monocarboxylate transporters (MCTs), cyclophilins, and integrins, to regulate metabolic pathways and immune cell activation.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,252.5	24.5	2.0
2	8	317.0	8.4	2.6
3	8	155.7	6.0	3.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,420.2	176.8	12.4
2	16	329.7	39.4	12.0
3	16	150.4	17.4	11.6

RECOVERY

The recovery of mouse CD147 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:320	107	100-120
Lysate	1:80	92	81-112
	1:160	92	82-100

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:20. The cell lysate was initially diluted 1:800. )

		Mouse serum(Sample Diluent PT 1)	Lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	98	101
	Range (%)	82-114	92-109
1:8	Average% of Expected	89	106
	Range (%)	74-105	95-118
1:16	Average% of Expected	81	97
	Range (%)	76-86	95-99

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse CD147 produced at Sunlong Medical™.