DESCRIPTION

Tetranectin (TN), which is encoded by the Clec3b gene, is a homotrimeric molecule of the C-type superfamily of lectins. TN was initially identified as a plasminogen-binding protein from human plasma, which shares an 87% amino acid sequence identity to its counterpart in mice. TN mRNA is detected in various tissues, but its protein expression is more tissue restricted. Several functions of TN have been reported including regulation of fibrinolysis and proteolytic processes, osteogenesis, myogenesis, Parkinson disease, and bone mineralization.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,486.2	84.6	3.4
2	8	666.3	60.5	9.1
3	8	344.8	32.0	9.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,548.4	197.2	7.7
2	16	674.7	53.3	7.9
3	16	344.1	34.1	9.9

RECOVERY

The recovery of human Tetranectin spiked to three different levels throughout the range of the assay in human plasma was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:32,000	96	90-101
	1:64,000	97	95-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:4,000.)

		Human plasma
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	99
	Range (%)	98-100
1:8	Average% of Expected	98
	Range (%)	97-99
1:16	Average% of Expected	102
	Range (%)	100-104

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Tetranectin produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)