DESCRIPTION

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail. L1CAM is primarily expressed in the nervous system and is involved in neuronneuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts. L1CAM is overexpressed in many human cancers and is often associated with bad prognosis.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	3,990.4	130.9	3.3
2	8	959.0	86.4	9.0
3	8	496.8	38.9	7.8

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	3,984.7	249.4	6.3
2	16	950.4	71.2	7.5
3	16	489.4	29.4	6.0

RECOVERY

The recovery of human L1CAM spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Humam plasma	1:64	94	92-96
	1:128	99	97-101
Cell lysate	1:320	93	91-94
	1:640	93	90-96

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:8. The cell lysate was initially diluted 1:40.)

		Human plasma(Sample Diluent PT 4B1)	Cell lysate(Sample Diluent PT 4B1-ef)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	107	107
	Range (%)	105-108	99-115
1:8	Average% of Expected	117	118
	Range (%)	108-126	110-126
1:16	Average% of Expected	120	123
	Range (%)	110-129	121-126

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human L1CAM produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)