DESCRIPTION

DDR1 (Discoidin Domain Receptor 1) is a receptor tyrosine kinase (RTK) that belongs to the DDR family. It binds to extracellular matrix (ECM) components such as collagen through its extracellular discoidin domain, activates intracellular tyrosine kinase activity, and regulates processes including cell proliferation, migration, differentiation, and apoptosis. DDR1 plays important roles in various physiological and pathological processes, including tissue repair, inflammatory responses, and tumorigenesis. In oncology, the abnormal expression of DDR1 is associated with the invasiveness and metastatic potential of various cancers, making it a potential therapeutic target. Additionally, DDR1 is involved in the progression of cardiovascular and neurodegenerative diseases. Studying the signaling pathways and functional mechanisms of DDR1 helps to develop new therapeutic strategies for the treatment of cancer, cardiovascular diseases, and neurodegenerative diseases.

PRINCIPLE OF THE ASSAY

An anti-tag antibody is pre-coated onto the bottom of wells. After adding antigen or samples, Tagged-Capture antibody and HRP-conjugated detection antibody, a sandwich complex is formed in the solution. TMB acts as a HRP substrate, and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns the solution yellow. The color intensity is proportional to the quantity of bound protein, which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,005.7	21.2	1.1
2	8	511.3	8.5	1.7
3	8	264.9	9.6	3.6

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,043.7	70.7	3.5
2	16	513.7	10.9	2.1
3	16	265.0	6.9	2.6

RECOVERY

The recovery of human DDR1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:128	99	87-108
Cell culture supernatant	1:64	99	94-108
Cell lysate	1:256	82	77-86

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma was initially diluted 1:8. The cell culture supernatant was initially diluted 1:4. The cell lysate were initially diluted 1:16.)

		Human plasma	Cell culture supernatant	Cell lysate
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	105	101	97
	Range (%)	101-109	100-101	90-104
1:8	Average% of Expected	94	86	98
	Range (%)	90-99	74-97	97-98
1:16	Average% of Expected	102	89	110
	Range (%)	94-110	83-96	96-125

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human DDR1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)