DESCRIPTION

BST2 (Bone Marrow Stromal Cell Antigen 2), also known as Tetherin, CD317, or HM1.24, is a type II transmembrane glycoprotein widely involved in immunomodulation and antiviral defense. It restricts viral release and transmission by binding nascent viral particles to the host cell membrane. In addition, BST2 is highly expressed in a variety of human cancers and is closely associated with tumor cell proliferation, invasion, migration, and immune escape. For example, in laryngeal squamous cell carcinoma, high expression of BST2 correlates with poorer patient prognosis. BST2 is also involved in cell membrane integrity and lipid raft formation, and can activate the epidermal growth factor receptor signaling pathway, thus playing a role in tumorigenesis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,979.3	198.3	10.0
2	8	554.4	17.0	3.1
3	8	268.3	17.6	6.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,959.7	213.2	10.9
2	16	499.9	58.2	11.6
3	16	276.0	17.2	6.2

RECOVERY

The recovery of mouse BST2 spiked to three different levels throughout the range of the assay in mouse serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:2	75	73-78
	1:4	81	80-84

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Mouse serum
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	113
	Range (%)	110-116
1:8	Average% of Expected	86
	Range (%)	79-93

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant mouse BST2 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)