DESCRIPTION

ANGPTL3 is a secreted glycoprotein primarily synthesized by hepatocytes and belongs to the angiopoietin-like protein family. ANGPTL3 plays a critical role in both biological processes, such as lipid metabolism, angiogenesis, and hematopoietic function, and pathological changes, including atherosclerosis, carcinogenesis, nephrotic syndrome, diabetes, liver diseases and so on. ANGPTL3 inhibits low-density lipoprotein (LDL) and endothelial lipase (EL), two enzymes that hydrolyze triglycerides and phospholipids in plasma lipoproteins. ANGPTL3-deficient mice showed low plasma HDL cholesterol and HDL phospholipid (PL). ANGPTL3 can also regulate triglyceride metabolism by inhibiting lipoprotein lipase (LPL). ANGPTL3-deficient mice exhibit hypotriglyceridemia and elevated LPL activity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	1,136.9	43.9	3.9
2	20	249.8	4.3	1.7
3	20	63.7	2.0	3.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	983.4	89.1	9.1
2	24	239.4	11.0	4.6
3	24	62.0	1.9	3.0

RECOVERY

The recovery of mouse Angiopoietin-like 3/ANGPTL3 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Mouse serum	1:4,000	93	86-98
	1:8,000	100	94-107
Cell culture supernatant	1:2	93	86-103
	1:4	92	83-103

LINEARITY

To assess the linearity of the assay, mouse serum and cell culture supernatant samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The mouse serum was initially diluted 1:500.)

		Mouse serum	Cell culture supernatant
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	100	108
	Range (%)	99-101	105-111
1:8	Average% of Expected	100	103
	Range (%)	97-102	103-104
1:16	Average% of Expected	101	101
	Range (%)	101-102	96-105

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Mouse Angiopoietin-like 3/ANGPTL3 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)