DESCRIPTION

PepsinogenⅠand Ⅱ, as precursors of pepsin, are produced by gastric mucosa and released into the gastric lumen and peripheral circulation. The advanced inflammation of gastric mucosa and its progression toward atrophic gastritis (AG) in the corpus is associated with a change in serum biomarkers pepsinogenⅠand Ⅱ.(PMID:23496362). PepsinogenⅡ also known as progastricsin or PGC (pepsinogen C), is an aspartic protease expressed primarily in gastric chief cells and is a novel marker of type 2 cells with advantages over many of the current markers used to identify type 2 cells in the developing lung(PMID:14578117). It is involved in proteolysis and peptidolysis.

PRINCIPLE OF THE ASSAY

A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody also binds to the analyte. Horseradish peroxidase (HRP)-conjugated secondary antibody binds to the detection antibody. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	5.4	0.2	4.3
2	20	25.2	0.9	3.4
3	20	89.9	6.0	6.7

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	5.6	0.2	3.1
2	24	24.4	0.6	2.4
3	24	80.8	4.9	6.1

RECOVERY

The recovery of human PGⅡ spiked to three different levels throughout the range of the assay in various matrices was evaluated. (The human plasma samples were initially diluted 1:2)

Sample Type		Average% of Expected	Range (%)
Human plasma	1:2	97	75-128
	1:4	100	77-128
Cell culture supernatant	1:2	88	88-89
	1:4	95	86-101

LINEARITY

To assess the linearity of the assay, three samples were spiked with high concentrations of human PGⅡ in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human plasma samples were initially diluted 1:2)

		Human plasma(Sample Diluent PT 3-ec)	Cell culture supernatant(Sample Diluent PT 1-ef)
1:2	Average% of Expected	106	85
	Range (%)	79-118	85-89
1:4	Average% of Expected	101	91
	Range (%)	90-113	84-99
1:8	Average% of Expected	93	99
	Range (%)	83-100	92-106
1:16	Average% of Expected	100	105
	Range (%)	93-108	104-106

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PGⅡ Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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• Price: USD, per kit (48T / 96T options available)