DESCRIPTION

ZFP36L2 (Zinc Finger Protein 36-Like 2) is an RNA-binding protein of the tristetraprolin (TTP) family, characterized by two CCCHtype zinc-finger domains that selectively bind AU-rich elements (AREs) within target mRNAs. Through recruitment of the CCR4NOT deadenylase complex, ZFP36L2 promotes mRNA degradation, thereby exerting post-transcriptional control over genes involved in cell cycle progression, immune regulation, and hematopoietic differentiation. ZFP36L2 is expressed in diverse tissues, with notable roles in lymphoid development, T cell quiescence, and B cell maturation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	13.19	0.63	4.78
2	8	3.26	0.08	2.42
3	8	1.43	0.04	2.73

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	12.68	0.75	5.89
2	16	3.16	0.14	4.45
3	16	1.50	0.09	5.71

RECOVERY

The recovery of human ZFP36L2 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	99	92-104
	1:32	105	101-108

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	127
	Range (%)	125-130
1:8	Average% of Expected	118
	Range (%)	116-120
1:16	Average% of Expected	91
	Range (%)	89-94

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human ZFP36L2 Sandwich ELISA Kit produced at Sunlong Medical™.