DESCRIPTION

USP33 (Ubiquitin Specific Protease 33) is an important member of the deubiquitinating enzyme (DUB) family. It regulates a variety of cellular processes by removing ubiquitin chains from substrate proteins and preventing proteasomal degradation. USP33 plays a significant role in multiple cellular activities, including thyroid hormone activation, β2-adrenergic receptor signaling, development of the neural axis in vertebrates, immunity and autophagy, centrosome duplication, and tumor cell migration. Additionally, USP33 is involved in the regulation of mitophagy by removing K63 ubiquitin chains from Parkin protein, thereby controlling the occurrence of mitophagy. In tumor progression, USP33 promotes tumor growth and metastasis by enhancing TGF-β signaling through deubiquitination and stabilization of TGFBR2 protein.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.05	0.13	2.65
2	8	1.32	0.05	3.64
3	8	0.60	0.02	2.87

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.17	0.30	5.89
2	16	1.30	0.04	3.42
3	16	0.62	0.03	4.49

RECOVERY

The recovery of human USP33 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:8	96	92-101
	1:16	108	101-116

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	96
	Range (%)	88-102
1:8	Average% of Expected	90
	Range (%)	82-102
1:16	Average% of Expected	112
	Range (%)	101-123

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human USP33 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)