DESCRIPTION

Thymic stromal lymphopoietin (TSLP) is a multifunctional cytokine primarily produced by epithelial cells in response to tissue damage, infection, or allergen exposure. It plays a significant role in immune regulation, inflammation, and cancer. TSLP acts through its receptor complex, composed of TSLP receptor (TSLPR) and interleukin-7 receptor alpha chain (IL-7Rα), to activate downstream signaling pathways, including JAK-STAT, PI3K-AKT, and MAPK. TSLP is involved in various physiological and pathological conditions, including asthma, allergic diseases, and chronic inflammatory diseases. It promotes type 2 immune responses by inducing the release of T cell-attracting chemokines from monocytes and enhancing the maturation of CD11c(+) dendritic cells. Additionally, TSLP plays a role in tissue homeostasis, host defense, and microbiome regulation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	210.6	2.9	1.4
2	8	95.6	1.7	1.8
3	8	65.0	2.2	3.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	204.1	8.2	4.0
2	16	95.4	2.1	2.2
3	16	64.7	2.1	3.2

RECOVERY

The recovery of human TSLP spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	76	75-78
	1:4	111	103-120
Cell culture supernatant	1:2	119	114-123
	1:4	122	116-126

LINEARITY

To assess the linearity of the assay, human serum and cell culture supernatant samples were spiked with high concentrations of human TSLP and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum	Cell culture supernatant
1:2	Average% of Expected	80	103
	Range (%)	70-92	102-103
1:4	Average% of Expected	92	108
	Range (%)	84-101	105-111
1:8	Average% of Expected	101	108
	Range (%)	98-105	102-115
1:16	Average% of Expected	115	106
	Range (%)	109-123	102-111

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TSLP produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)