DESCRIPTION

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family. It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains. Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites. In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer. Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	40.52	2.15	5.31
2	20	8.74	0.33	3.74
3	20	2.24	0.12	5.50

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	41.01	2.66	6.48
2	24	8.76	0.25	2.84
3	24	2.28	0.16	7.13

RECOVERY

The recovery of human TNC/Tenascin-C spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human plasma	1:2	82	73-94
	1:4	95	82-113
Cell lysate	1:8	97	95-99
	1:16	102	97-110

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human plasma(Sample Diluent PT 1)	Cell lysate(Sample Diluent PT 3)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	92	96
	Range (%)	90-93	93-99
1:8	Average% of Expected	75	97
	Range (%)	74-75	96-98
1:16	Average% of Expected	99	109
	Range (%)	82-115	104-114

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human TNC/Tenascin-C Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)