DESCRIPTION

Metalloproteinase inhibitor 1 (TIMP1) is also named as Erythroid-potentiating activity (EPA), Fibroblast collagenase inhibitor (Collagenase inhibitor) and CLGI. TIMP1 is one of the tissue inhibitor members of the metalloproteinase family. TIMP1 regulates the balance of matrix remodeling during the degradation of extracellular matrix by inhibiting the proteolytic activity of matrix metalloproteinases (MMPs). TIMP1 promotes the occurrence and development of GC, which is consistent with previous reports about TIMP1 as an oncogene in other cancers, including breast cancer, colon cancer, glioblastoma, and non-small cell lung cancer. TIMP1 is reportedly as a tumor-secreted protein that could be detected in the serum. In a diabetic environment, TIMP1 initially binds to TFRC, leading to increased TFRC expression, thereby heightening osteoblast sensitivity to ferroptosis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,055.6	32.5	3.1
2	8	256.9	9.8	3.8
3	8	129.8	6.1	4.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,016.6	53.0	5.2
2	16	255.2	10.0	3.9
3	16	130.4	4.8	3.7

RECOVERY

The recovery of human TIMP1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:3,200	101	97-103
	1:6,400	105	100-109
Cell culture supernatant	1:80	108	105-110
	1:160	112	108-114
Saliva	1:800	104	101-107
	1:1,600	107	105-111

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum and saliva were initially diluted 1:400. The cell culture supernatant was initially diluted 1:10.)

		Human serum	Cell culture supernatant	Saliva
1:2	Average% of Expected	100	100	100
	Range (%)	-	-	-
1:4	Average% of Expected	118	105	99
	Range (%)	110-125	103-108	93-105
1:8	Average% of Expected	121	107	98
	Range (%)	120-122	105-109	97-99
1:16	Average% of Expected	119	105	94
	Range (%)	115-123	103-106	93-94

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human TIMP1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)