DESCRIPTION

STAU1 (Staufen Double-Stranded RNA Binding Protein 1) is a highly conserved multifunctional double-stranded RNA binding protein that is widely involved in a variety of cellular processes. STAU1 is involved in the transport of mRNA from the nucleus to the cytoplasm. In neurons, STAU1 promotes the transport and translation of mRNA by binding to the 3' untranslated region (3'UTR) of mRNA. STAU1 inhibits the retention of these mRNAs in the nucleus by binding to the inverted repeat Alu element (IRAlus) on mRNA, thereby enhancing its nuclear export; STAU1 also induces translation-dependent mRNA degradation (SMD) through direct interaction with UPF1, a process that is critical for gene expression regulation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	12.23	0.45	3.67
2	8	3.09	0.13	4.13
3	8	1.55	0.12	7.49

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	12.28	0.46	3.74
2	16	3.07	0.24	7.97
3	16	1.58	0.11	6.72

RECOVERY

The recovery of human STAU1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:32	112	109-113

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	120
	Range (%)	110-130
1:8	Average% of Expected	95
	Range (%)	74-108
1:16	Average% of Expected	85
	Range (%)	77-93

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human STAU1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)