DESCRIPTION

Specificity Protein 3 (SP3) is a transcription factor belonging to the Sp1-related gene family. It regulates gene transcription by binding to GC and GT boxes in the regulatory elements of target genes. The SP3 protein contains a zinc-finger DNA-binding domain and multiple transcriptional activation domains, functioning as a dual-function transcription factor that can both activate and repress gene expression, depending on the cell type, target gene, and the presence of co-regulators. SP3 plays a crucial role in biological processes such as cell proliferation, differentiation, apoptosis, and embryonic development. Its abnormal expression is associated with various diseases, including Alzheimer's disease, breast cancer, and colorectal cancer. In some cases, SP3 may disrupt cell cycle regulation or inhibit apoptosis by competitively inhibiting the functions of other Sp family members (such as SP1). Additionally, SP3 is involved in the regulation of angiogenesis-related gene expression.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.04	0.06	1.15
2	8	1.43	0.03	2.29
3	8	0.67	0.03	3.74

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.28	0.26	4.99
2	16	1.34	0.11	7.85
3	16	0.67	0.02	3.28

RECOVERY

The recovery of human SP3 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:16	93	84-99

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:4.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	82
	Range (%)	73-90
1:8	Average% of Expected	84
	Range (%)	72-97
1:16	Average% of Expected	102
	Range (%)	94-108

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human SP3 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)