DESCRIPTION

Spalt-Like Transcription Factor 4 (SALL4) is a zinc-finger transcriptional factor for embryonic stem cell self-renewal and pluripotency. SALL4 overexpression is found in several cancer tissues and hematologic malignancies, suggesting a key role of SALL4 in cancer onset and progression. Reactivation of SALL4 in cancerous cells is a key event in tumorigenesis and confers malignancy features to cells. The SALL4 protein contains several zinc finger cluster (ZFC) domains spread in different parts of the protein which give it both transcription activation and repression functions. SALL4 has two isoforms. SALL4A originates from full length transcript, while SALL4B as a spliced isoform lacks a part of exon 2. SALL4 abundancy is regulated by transcriptional and post-transcriptional mechanisms. Both SALL4A and SALL4B isoforms can bind β-catenin protein and synergistically activate the Wnt/β-catenin signaling pathway.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	1,220.4	72.4	5.9
2	20	336.6	22.0	6.5
3	20	74.8	4.41	5.9

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	1,252.4	111.4	8.9
2	24	328.6	18.9	5.8
3	24	74.6	6.2	8.3

RECOVERY

The recovery of human SALL4 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	110	95-128
	1:4	98	77-127

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum
1:2	Average% of Expected	83
	Range (%)	81-85
1:4	Average% of Expected	91
	Range (%)	86-96
1:8	Average% of Expected	94
	Range (%)	90-98
1:16	Average% of Expected	92
	Range (%)	91-92

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human SALL4 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)