DESCRIPTION

RAD21 (also known as KIAA0078, NXP1, HR21, Mcd1, Scc1), plays a pivotal role in various cellular processes, including DNA repair, chromosome segregation, and gene expression regulation. It is a structural component of the cohesin complex, which consists of RAD21, SMC1a, SMC3, and SCC3 proteins. This complex is essential for maintaining sister chromatid cohesion during cell division, ensuring accurate chromosome segregation and preventing inappropriate recombination between repetitive DNA regions. Mutations in RAD21 have been linked to several human genetic disorders, collectively termed cohesinopathies. These include Cornelia de Lange syndrome (CdLS), a developmental disorder characterized by intellectual disability, distinctive facial features, and growth retardation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.20	0.18	3.49
2	8	1.31	0.04	2.79
3	8	0.64	0.02	3.21

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.27	0.16	2.97
2	16	1.24	0.08	6.65
3	16	0.62	0.03	5.42

RECOVERY

The recovery of human RAD21 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:8	93	89-99
	1:16	94	89-101

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	105
	Range (%)	82-119
1:8	Average% of Expected	94
	Range (%)	77-128
1:16	Average% of Expected	89
	Range (%)	73-114

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human RAD21 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)