DESCRIPTION

PON1 (Paraoxonase 1) is a serum enzyme primarily synthesized in the liver and associated with high-density lipoproteins (HDL). It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 plays a crucial role in detoxifying organophosphate compounds and has antioxidant properties that protect against oxidative stress. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	12.37	0.72	5.86
2	8	2.80	0.14	4.85
3	8	1.46	0.08	5.47

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	11.98	0.88	7.38
2	16	2.84	0.14	4.91
3	16	1.42	0.08	5.41

RECOVERY

The recovery of human PON1 spiked to three different levels throughout the range of the assay in human serum was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:640	108	103-111
	1:1,280	108	104-113

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:40.)

		Human serum
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	116
	Range (%)	112-120
1:8	Average% of Expected	115
	Range (%)	112-117
1:16	Average% of Expected	113
	Range (%)	111-114

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PON1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)