DESCRIPTION

PNMT (Phenylethanolamine N-methyltransferase) is the final enzyme in the catecholamine synthesizing cascade that converts noradrenaline to epinephrine by transferring a methyl group from S-adenosyl-L-methionine (SAM) to norepinephrine. PNMT is primarily found in the adrenal medulla, where it is responsible for the production of epinephrine. It is also present in certain areas of the brain, such as the amygdala and retina, where it may be involved in various neurophysiological functions. Variations in the PNMT gene have been associated with Alzheimer's disease, hypertension, and other disorders.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	20	10.43	0.37	3.54
2	20	2.59	0.09	3.51
3	20	0.62	0.04	6.95

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	24	9.95	0.54	5.41
2	24	2.54	0.12	4.56
3	24	0.70	0.04	5.54

RECOVERY

The recovery of human PNMT spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:4	80	73-86
	1:8	93	93-94

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	84
	Range (%)	77-90
1:8	Average% of Expected	82
	Range (%)	72-93
1:16	Average% of Expected	75
	Range (%)	74-75

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PNMT Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)