DESCRIPTION

PLSCR1 (Phospholipid scramblase 1) is a member of the family of membrane proteins that mediate the transbilayer movement of phospholipids ("scrambling") in a Ca2+ dependent manner, which was identified as an a type II transmembrane protein involved in the calcium-dependent, nonspecific, rapid redistribution of phospholipids. A cysteine-rich palmitoylation motif [184CCCPCC189] of PLSCR1, a thioester linkage to the sulfhydryl groups of cysteines, controls the transportation of PLSCR1 to the cell membrane or nucleus. PLSCR1 is strongly expressed in response to interferon (IFN) treatment and viral infection.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.67	0.22	3.83
2	8	1.39	0.05	3.87
3	8	0.65	0.04	5.51

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	6.02	0.48	7.97
2	16	1.36	0.06	4.21
3	16	0.69	0.05	7.07

RECOVERY

The recovery of human PLSCR1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Urine	1:16	94	89-100
	1:32	94	90-98
Lysate	1:256	93	88-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate was initially diluted 1:16.)

		Urine	Lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	108	105
	Range (%)	104-113	99-110
1:8	Average% of Expected	115	115
	Range (%)	112-119	106-123
1:16	Average% of Expected	113	104
	Range (%)	108-115	88-129

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human PLSCR1 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)