DESCRIPTION

The mitochondrial serine/threonine-protein kinase PINK1, also known as BRPK and PARK6, protects cells from mitochondrial stress-induced dysfunction. PINK1 performs many important cell functions and has been highlighted for its role in early-onset Parkinson's disease (PD). PINK1/Parkin-mediated mitophagy halts the accumulation of toxic mitochondrial products that can cause neuronal loss in PD and prevents damaged mitochondria from fusing with the healthy network. Moreover, both PINK1 and Parkin have also been shown to function in a quality control pathway parallel to mitophagy by facilitating piecemeal degradation of mitochondria via the formation of mitochondria-derived vesicles (MDVs).

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	4.41	0.20	4.53
2	8	1.08	0.05	4.37
3	8	0.58	0.02	2.86

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	4.44	0.24	5.44
2	16	1.08	0.06	5.60
3	16	0.57	0.02	3.86

RECOVERY

The recovery of human PINK1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:256	81	70-92

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:16. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	106
	Range (%)	94-118
1:8	Average% of Expected	94
	Range (%)	91-97
1:16	Average% of Expected	94
	Range (%)	92-96

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PINK1 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)