DESCRIPTION

PlGF (placenta growth factor), also named as PGF, is an angiogenic factor which belongs to vascular endothelial growth factor (VEGF) family. The human PlGF gene is located on chromosome 14q14 and encodes 4 isoforms of PlGF. The protein is secreted as a glycosylated homodimer and PlGF-1 and -3 are diffusible isoforms whereas PlGF-2 and PlGF-4 have heparin binding domains. PlGF was originally identified in the placenta, where it regulates growth and differentiation of trophoblasts. It is also expressed in umbilical vein endothelial cells and other non-placental tissues, like the thyroid gland and developing lung tissue. PlGF has angiogenic properties, enhancing survival, growth and migration of endothelial cells in vitro, and promotes vessel formation in certain in-vivo models. Serum levels of PIGF and sFlt-1 (also known as soluble VEGF receptor-1) are altered in women with preeclampsia. PIGF is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	487.9	36.1	7.4
2	20	124.0	4.3	3.5
3	20	28.1	2.4	8.5

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	505.5	37.3	7.4
2	24	124.0	8.3	6.7
3	24	29.2	2.7	9.2

RECOVERY

The recovery of human PGF/PIGF spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	106	95-117
	1:4	114	97-126
Cell culture supernatant	1:2	101	94-119
	1:4	103	98-108
Urine	1:2	96	86-106
	1:4	95	90-99

LINEARITY

To assess the linearity of the assay, human serum samples were spiked with high concentrations of human PGF/PIGF and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Cell culture supernatant and urine samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell culture supernatant was initially diluted 1:2. )

		Human serum(Sample Diluent PT 3)	Cell culture supernatant(Sample Diluent PT 4B1)	Urine(Sample Diluent PT 4B1)
1:2	Average% of Expected	92	100	100
	Range (%)	84-100	-	-
1:4	Average% of Expected	106	99	93
	Range (%)	98-113	87-106	90-96
1:8	Average% of Expected	111	103	91
	Range (%)	103-118	97-109	82-100
1:16	Average% of Expected	117	97	83
	Range (%)	106-123	92-103	80-85

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human PGF/PIGF Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

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