DESCRIPTION

Noggin is a secreted protein that belongs to the BMP antagonist family, and it has been found to play a complex and contextdependent role in cancer development (PMID: 38012621). Noggin has been shown to have anti-cancer effects by inhibiting the activity of BMPs, which are a group of proteins that belong to the TGF-β superfamily and are involved in various cellular processes such as cell proliferation, differentiation, and migration (PMID: 32486027). By blocking BMP activity, Noggin has been shown to inhibit the growth of osteolytic prostate cancer lesions (PMID: 16126463). In lung cancer cells, Noggin abrogated angiogenic activity enhanced by BMP-2 (PMID: 15037653). However, Noggin can also have pro-cancer effects in certain contexts. For example, in patients with gastric cancer, high Noggin expression is associated with a poor prognosis, and Noggin promotes the proliferation of gastric cancer cells by upregulating EGFR signaling (PMID: 32705152).

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	4,879.9	340.2	7.0
2	8	1,113.2	52.3	4.7
3	8	530.9	26.5	5.0

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	5,059.9	423.9	8.4
2	16	1,105.2	55.0	5.0
3	16	533.7	28.9	5.4

RECOVERY

The recovery of human/mouse/rat Noggin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:2	72	72-73
Tissue lysate	1:8	73	71-74
	1:16	84	80-91

LINEARITY

To assess the linearity of the assay, human serum samples was spiked with high concentrations of Noggin and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. Tissue lysate samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum	Tissue lysate
1:2	Average% of Expected	87	100
	Range (%)	74-99	-
1:4	Average% of Expected	87	96
	Range (%)	81-93	89-102
1:8	Average% of Expected	95	103
	Range (%)	93-97	100-106
1:16	Average% of Expected	102	130
	Range (%)	101-102	129-131

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat Nogginin produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)