DESCRIPTION

Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites. Multiple high molecular weight and low molecular weight MAP2 isoforms are expressed within axons, dendrites, and cell bodies. MAP2 isoforms differ in their tissue and developmental expression pattern. In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis. In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells. MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,351.2	30.6	2.3
2	8	435.0	20.3	4.7
3	8	172.1	5.9	3.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,334.1	42.5	3.2
2	16	436.6	16.0	3.7
3	16	177.2	11.0	6.2

RECOVERY

The recovery of MAP2 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:8	106	103-111
	1:16	112	102-126
Lysate	1:8	101	84-128

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

		Human serum(Sample Diluent PT 4)	Lysate(Sample Diluent PT 3)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	98	98
	Range (%)	76-116	78-112
1:8	Average% of Expected	84	93
	Range (%)	73-104	71-115
1:16	Average% of Expected	90	-
	Range (%)	74-118	-

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat MAP2 produced at Sunlong Medical™.

Ordering & shipping

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