DESCRIPTION

GSR (glutathione-disulfide reductase) is also named as glutathione reductase (GR), GLUR, GRD1, which is an enzyme that catalyzes the reduction of glutathione disulfide (GSSG) to the sulfhydryl form of glutathione (GSH), which is a critical molecule in resisting oxidative stress and maintaining the reducing environment of the cell. Glutathione plays a key role in maintaining proper function and preventing oxidative stress by acting as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces H2O2, a dangerously reactive species within the cell. In addition, GSH plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, and regenerates antioxidants such as vitamins C and E to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in maintaining the oxidative cell balance, where it is critical that a cell maintains high levels of the reduced glutathione and a low level of oxidized glutathione disulfide. This narrow balance is "buffered" by GSR, which catalyzes the reduction of GSSG to GSH.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	9.29	0.24	2.63
2	8	3.18	0.07	2.17
3	8	1.84	0.06	3.42

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	8.76	0.62	7.06
2	16	2.95	0.25	8.32
3	16	1.82	0.06	3.18

RECOVERY

The recovery of human/mouse/rat GSR spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Lysate	1:16	81	71-90

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate was initially diluted 1:8. )

		Lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	114
	Range (%)	108-119
1:8	Average% of Expected	99
	Range (%)	80-119
1:16	Average% of Expected	98
	Range (%)	91-106

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse/rat GSR produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)