DESCRIPTION

DCC, also named as IGDCC1, belongs to the immunoglobulin superfamily and DCC family. DCC is a receptor for netrin required for axon guidance. DCC mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. DCC also acts as a dependence receptor required for apoptosis induction when not associated with the netrin ligand.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	4.72	0.13	2.67
2	8	1.19	0.04	3.07
3	8	0.67	0.02	2.95

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	4.60	0.19	4.07
2	16	1.21	0.04	3.29
3	16	0.63	0.05	7.85

RECOVERY

The recovery of human/mouse DCC spiked to three different levels throughout the range of the assay in lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Lysate	1:4	90	84-98
	1:8	95	91-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The lysate samples were initially diluted 1:16.)

		Lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	103
	Range (%)	100-107
1:8	Average% of Expected	109
	Range (%)	106-112
1:16	Average% of Expected	114
	Range (%)	109-119

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human/mouse DCC produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)