DESCRIPTION

MNT (MAX network transcriptional repressor ), also known as MAD6, ROX, bHLHd3 (class D basic helix-loop-helix protein 3) or MXD6, is a 582 amino acid nuclear protein. Mnt contains one basic helix-loop-helix (bHLH) domain, and Mnt acts primarily as a transcriptional repressor. It binds to the Max(Myc-associated factor X) protein and inhibits the expression of target genes that are typically activated by Myc. This repressive action is crucial in maintaining the balance between cell growth and apoptosis, preventing uncontrolled cell proliferation. It predicts that antagonism of Myc by Mxd-family proteins and Mnt plays significant roles in regulating the various activities of Myc, including its role in tumorigenesis.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	2,442.9	105.8	4.3
2	8	514.9	29.1	5.6
3	8	147.1	3.5	2.4

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	2,400.0	145.5	6.1
2	16	509.7	21.2	4.2
3	16	143.5	5.5	3.8

RECOVERY

The recovery of human MNT spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:1,280	87	79-94

LINEARITY

To assess the linearity of the assay, cell lysate samples were spiked with high concentrations of human MNT and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:80.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	99-101
1:4	Average% of Expected	96
	Range (%)	92-100
1:8	Average% of Expected	96
	Range (%)	80-111
1:16	Average% of Expected	100
	Range (%)	77-112

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human MNT Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)