DESCRIPTION

Minichromosome maintenance protein 3 (MCM3) is a key component of the MCM protein family, which plays a crucial role in the initiation and regulation of DNA replication. MCM3 is highly conserved across species and is essential for maintaining genomic stability. In recent years, research has shown that MCM3 is overexpressed in various cancers, including hepatocellular carcinoma (HCC), ovarian cancer, and medulloblastoma. MCM3 is involved in multiple biological processes, such as cell cycle regulation and cell proliferation. Functional enrichment analysis suggests that MCM3 is mainly involved in cell cycle and cell metabolic pathways. Furthermore, MCM3 is involved in the formation of pre-replicative complexes and is regulated by phosphorylation during the cell cycle.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	6.39	0.27	4.29
2	8	1.50	0.09	5.99
3	8	0.68	0.03	4.30

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.90	0.56	9.44
2	16	1.39	0.14	9.79
3	16	0.66	0.05	6.93

RECOVERY

The recovery of human MCM3 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:64	101	90-122

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:16.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	103
	Range (%)	102-104
1:8	Average% of Expected	108
	Range (%)	105-111
1:16	Average% of Expected	115
	Range (%)	113-117

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human MCM3 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)