DESCRIPTION

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R is ubiquitously expressed in macrophages, microglia, osteoclasts, and myeloid dendritic cells, but is rarely expressed in hematopoietic stem cells.CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	1,819.3	110.5	6.1
2	8	364.3	8.3	2.3
3	8	194.7	4.5	2.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	1,782.6	107.4	6.0
2	16	365.2	8.0	2.2
3	16	187.7	9.3	5.0

RECOVERY

The recovery of human CSF1R/CD115 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:320	106	93-113
	1:640	101	95-106
Cell lysate	1:64	95	92-101
	1:128	96	94-98

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:80. The cell lysate was initially diluted 1:8. )

		Human serum	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	104	99
	Range (%)	102-105	91-107
1:8	Average% of Expected	101	105
	Range (%)	99-102	91-118
1:16	Average% of Expected	97	113
	Range (%)	89-110	99-127

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CSF1R/CD115 Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
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• Price: USD, per kit (48T / 96T options available)