DESCRIPTION

CPLX1 (Complexin I) is a small synaptic protein that plays an important role mainly in the central nervous system and is involved in the release of neurotransmitters and the functional regulation of synapses. CPLX1 is a member of the complexin/Synaphin family. CPLX1 plays a key role in synaptic vesicular exocytosis, and dysfunction of CPLX1 is associated with developmental and epileptic encephalopathy.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.41	0.07	1.29
2	8	1.29	0.05	3.88
3	8	0.59	0.03	5.08

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.25	0.20	3.81
2	16	1.27	0.05	3.94
3	16	0.60	0.03	5.00

RECOVERY

The recovery of human CPLX1 spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:128	101	96-106
	1:256	103	101-106

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:32.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	97
	Range (%)	95-99
1:8	Average% of Expected	101
	Range (%)	94-108
1:16	Average% of Expected	96
	Range (%)	95-97

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CPLX1 Sandwich ELISA Kit produced at Sunlong Medical™.