DESCRIPTION

CD99 is a highly O-glycosylated type I transmembrane protein that is mainly expressed in cells of the hematopoietic system and at the intercellular borders of endothelial cells. CD99 is essential for transendothelial migration (TEM) of monocytes, neutrophils, and lymphocytes. It is frequently overexpressed in many types of tumors, particularly pediatric tumors including Ewing sarcoma and specific subtypes of leukemia. CD99 is also a novel marker of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) stem cells.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	10.03	0.35	3.54
2	8	2.71	0.16	5.92
3	8	1.39	0.09	6.56

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	10.00	0.44	4.36
2	16	2.68	0.17	6.37
3	16	1.41	0.11	7.99

RECOVERY

The recovery of human CD99 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Urine	1:80	104	99-112
Cell lysate	1:320	89	87-93

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The urine was initially diluted 1:2.5. The cell lysate was initially diluted 1:20.)

		Urine	Cell lysate
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	102	110
	Range (%)	98-107	108-112
1:8	Average% of Expected	107	113
	Range (%)	97-117	109-116
1:16	Average% of Expected	110	117
	Range (%)	94-127	110-124

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD99 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)