DESCRIPTION

CD79b is a member of the Ig-Superfamily. It is a crucial component of the B cell antigen receptor (BCR) complex, essential for B cell activation and function. CD79b forms a heterodimer with CD79a (Igα) and is involved in BCR signaling, which is critical for B cell development, proliferation, and differentiation. The N-terminal tyrosine kinase structure domain of CD79b can recruit and activate downstream signaling molecules such as Syk, playing a core role in B cell signaling. Moreover, mutations in CD79b can lead to constitutive activation of downstream signaling pathways, contributing to B cell lymphoma development.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	112.9	2.9	2.5
2	8	28.2	0.7	2.4
3	8	14.3	0.5	3.6

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	108.5	5.2	4.8
2	16	29.3	1.3	4.4
3	16	14.7	0.6	4.1

RECOVERY

The recovery of human CD79b spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:320	89	85-94
	1:640	93	84-99

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:160. )

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	102
	Range (%)	95-110
1:8	Average% of Expected	105
	Range (%)	96-115
1:16	Average% of Expected	112
	Range (%)	104-121

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CD79b Sandwich ELISA Kit produced at Sunlong Medical™.