DESCRIPTION

CD72 is a regulatory receptor expressed on B cells that plays a crucial role in maintaining self-tolerance and modulating B cell responses. It is a member of the C-type lectin superfamily. CD72 contains an immune receptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic tail, which recruits the tyrosine phosphatase SHP-1 to regulate B cell receptor (BCR) signaling negatively. Additionally, elevated levels of soluble CD72 (sCD72) have been reported in the serum of SLE patients, correlating with disease severity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	517.7	15.8	3.1
2	8	115.2	2.8	2.4
3	8	60.2	2.8	4.7

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	512.7	17.4	3.4
2	16	119.1	6.9	5.8
3	16	59.5	3.1	5.3

RECOVERY

The recovery of human CD72 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:4	91	84-97
	1:8	99	93-103
Cell lysate	1:128	106	99-113
	1:256	109	104-115

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:32. )

		Human serum(Sample Diluent PT 1)	Cell lysate(Sample Diluent PT 4B1)
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	108	104
	Range (%)	93-122	102-106
1:8	Average% of Expected	94	109
	Range (%)	89-103	105-115
1:16	Average% of Expected	103	106
	Range (%)	86-114	105-107

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD72 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)