DESCRIPTION

CD27 (also known as TNFRSF7) is a lymphocyte-specific member of the TNF/NGF-R family, which is found in NK, T, and B cell populations. CD27 is a T and B cell co-stimulatory molecule. Its activity is governed by the transient availability of its TNF-like ligand CD70 on lymphocytes and dendritic cells. This interaction may have a key role in controlling the size and function of antigen-primed lymphocyte populations. CD27 is also required for the effective generation and long-term maintenance of antigen-specific CD4+ and CD8+ T cell immunity.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	8	5.19	0.06	1.08
2	8	1.25	0.05	3.81
3	8	0.39	0.01	3.72

Inter-assay Precision

Sample	n	Mean (ng/mL)	SD	CV%
1	16	5.36	0.21	4.00
2	16	1.30	0.06	4.68
3	16	0.40	0.02	4.86

RECOVERY

The recovery of human CD27 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Average% of Expected	Range (%)
Human serum	1:160	99	79-111
Cell culture supernatant	1:32	98	84-112
	1:64	100	84-111

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The human serum was initially diluted 1:10. The cell culture supernatant was initially diluted 1:4. )

		Human serum	Cell culture supernatant
1:2	Average% of Expected	100	100
	Range (%)	-	-
1:4	Average% of Expected	94	91
	Range (%)	90-98	85-96
1:8	Average% of Expected	87	98
	Range (%)	79-95	77-118
1:16	Average% of Expected	87	95
	Range (%)	81-93	74-117

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human CD27 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)