DESCRIPTION

OX40 (CD134) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, OX40 is activated by its cognate ligand OX40L (CD134L) and functions as a T cell co-stimulatory molecule. OX40-OX40L interactions have been proposed as a potential therapeutic target for treating autoimmunity. OX40 can interact with TRAF2, TRAF3, and TRAF5. In addition to a membrane bound form, OX40 also exists as a soluble form, released by activated lymphocytes.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with biotin) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with biotin also binds to the analyte. Streptavidin-HRP binds to the biotin. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 20 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 24 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	20	79.1	2.8	3.6
2	20	290.5	9.1	3.1
3	20	1,270.6	67.0	5.3

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	24	68.9	5.8	8.4
2	24	278.3	25.4	9.1
3	24	1,144.6	59.9	5.2

RECOVERY

The recovery of human OX40/CD134 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type		Range (%)	Average% of Expected
Human serum	1:2	85-110	100
	1:4	84-102	96
Human plasma	1:2	101-118	111
	1:4	98-110	104

LINEARITY

To assess the linearity of the assay, human serum and plasma samples were spiked with high concentrations of OX40 in various matrices and diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample Type		Range (%)	Average% of Expected
Human serum	1:2	82-93	87
	1:4	78-89	84
	1:8	81-89	84
	1:16	84-89	87
Human plasma	1:2	98-104	101
	1:4	84-98	91
	1:8	92-96	94

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human OX40 produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD, per kit (48T / 96T options available)