DESCRIPTION

CCR2 (C-C motif chemokine receptor-2) is a transmembrane protein highly expressed in bone marrow and lymph nodes. It belongs to the G-protein-coupled seven-transmembrane receptor superfamily. CCR2 is the key functional receptor for MCP-1 (Monocyte chemoattractant protein-1). CCR2 and monocyte chemoattractant proteins (MCPs) play pivotal roles in the development of inflammatory responses and are crucial for the recruitment of immune cells to sites of inflammation. There are two isoforms of CCR2, CCR2a and CCR2b. CCR2a was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM.

PRINCIPLE OF THE ASSAY

Sandwich ELISA structure (Detection antibody labeled with HRP) A capture antibody is pre-coated onto the bottom of wells which binds to analyte of interest. A detection antibody labeled with HRP also binds to the analyte. TMB acts as the HRP substrate and the solution color will change from colorless to blue. A stop solution containing sulfuric acid turns solution yellow. The color intensity is proportional to the quantity of bound protein which is measurable at 450 nm with the correction wavelength set at 630 nm.

PRECISION

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested 8 times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in 16 separate assays to assess inter-assay precision.

Intra-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	8	539.9	17.5	3.2
2	8	117.9	3.0	2.6
3	8	51.6	2.6	5.1

Inter-assay Precision

Sample	n	Mean (pg/mL)	SD	CV%
1	16	519.1	30.0	5.8
2	16	116.1	4.3	3.7
3	16	51.2	3.1	6.1

RECOVERY

The recovery of human CCR2a spiked to three different levels throughout the range of the assay in cell lysate was evaluated.

Sample Type		Average% of Expected	Range (%)
Cell lysate	1:8	92	86-97
	1:16	101	88-117

LINEARITY

To assess the linearity of the assay, samples were diluted with the appropriate Sample Diluent to produce samples with values within the dynamic range of the assay. (The cell lysate was initially diluted 1:2.)

		Cell lysate
1:2	Average% of Expected	100
	Range (%)	-
1:4	Average% of Expected	94
	Range (%)	87-100
1:8	Average% of Expected	89
	Range (%)	78-101
1:16	Average% of Expected	88
	Range (%)	80-96

CALIBRATION

This immunoassay is calibrated against a highly purified recombinant human Human CCR2a Sandwich ELISA Kit produced at Sunlong Medical™.

Ordering & shipping

• Minimum order: 1 kit
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• Price: USD, per kit (48T / 96T options available)