Quantitative Strain of Escherichia coli - Basic Information

Recommended Medium	Peptone: 10.0g, Beef extract powder: 3.0g, Sodium chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1 (25°C)
Growth Conditions	37°C； 18-24h； Aerobic
Storage Conditions	-20°C
Morphology	Colony diameter 1-2mm, round, regular margin, opaque, grayish-white front, convex center, smooth surface, bright surface, moist texture, easy to pick up, G- (red), rod-shaped, purity: pure
Procedure	① First culture the strain into a bacterial suspension according to the culture conditions; ② Measure the absorbance of the bacterial suspension, and calculate the initial concentration of the bacterial suspension according to the standard formula; ③ The initial concentration multiplied by the survival rate is the actual concentration after lyophilization; ④ Dilute the bacterial suspension according to the actual concentration after lyophilization to obtain the target concentration, then aliquot and lyophilize;
Reference Use	Research； Analysis and detection. Strains for research and quality control
Sharing Method	Public welfare sharing