Escherichia coli Quantitative Strain - Basic Information

Recommended Medium	Peptone: 10.0g, Beef extract powder: 3.0g, Sodium chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Morphology	Colony diameter 1-2mm, round, regular margin, opaque, grayish-white front, raised center, smooth and shiny surface, moist texture, easy to pick, G- (red), rod-shaped, purity: pure
Procedure	① First culture the strain into a bacterial suspension according to the growth conditions; ② Measure the absorbance of the bacterial suspension and calculate the initial concentration according to the standard formula; ③ Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; ④ Dilute the bacterial suspension according to the actual post-lyophilization concentration to obtain the target concentration, then aliquot and lyophilize;
Reference Use	Research; Analysis and detection. Strain for research and quality control
Sharing Method	Public welfare sharing