Escherichia coli Quantitative Strain - Basic Information

medium	Peptone: 10.0g, Beef Extract Powder: 3.0g, Sodium Chloride: 5.0g, Agar: 15.0g, pH: 7.3±0.1 (25 C)
Growth Conditions	37 C; 18-24h; Aerobic
Storage Conditions	-20 C
Morphology	Size: 1-2mm, Shape: Round, Margin: Entire, Opacity: Opaque, Color: Grayish-white, Elevation: Convex, Surface: Bright and smooth, Texture: Moist and easy to pick
Procedure	First culture the strain into a bacterial suspension according to the culture conditions; Measure the absorbance of the bacterial suspension and calculate the initial concentration according to the standard formula; Multiply the initial concentration by the survival rate to obtain the actual concentration after lyophilization; Dilute the bacterial suspension based on the actual concentration after lyophilization to obtain the target concentration, then aliquot and lyophilize;
Reference Use	Research; Analysis and detection. Strains for research and quality control, positive control strain for《GB 4789.2-2010 Aerobic Plate Count》,《GB 4789.3-2010 Enumeration of Coliforms》, Research; Analysis and detection. Positive control strain for《GB/T 4789.32-2002 Rapid Detection of Coliform Bacteria》,《GB 4789.39-2013 Enumeration of Fecal Coliforms》, quality control strain of the 2015 edition of Chinese Pharmacopoeia.
Sharing Method	Public Welfare Sharing

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