TOP10 E. coli containing (pUC57-PA) - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (30℃ for molds, 37℃ for bacteria) with rapid shaking to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap and draw out all the dissolved liquid, then inoculate onto 1-2 agar plates (about 200 μL per plate; for anaerobic bacteria, the Recommended Medium should be placed in an anaerobic environment for 24h in advance to remove oxygen); ④ Spread evenly and culture under the above growth conditions.
Sharing Method	Public Welfare Sharing