DH5α (containing pCMV-MCS1-EGFP-CAG-MCS2-Neo plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18–24 h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Wipe the exterior of the cryovial with an alcohol swab for disinfection, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, remove all of the dissolved liquid, and inoculate onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Place the antibiotic-resistant plates under the specified conditions above and incubate for 18–24 h before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic-resistant Recommended Medium, then culture overnight on a shaker at 37°C; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing

Ordering & shipping

• Minimum order: 1 unit
• Lead time: varies with order quantity and specifications. Please consult your local distributor or our sales team for confirmation
• Payment: T/T 100% in advance; PayPal; Alibaba Trade Assurance
• Shipping: FedEx / DHL / UPS express; cold-chain packing available on request
• Price: USD; specification / size is listed on this page