Escherichia coli DH5α(pLV3-4XIRF5-miniP-tdTomato-4×ISRE-miniP-mTagBFP2-Blast)
Stbl3（含pLV3-4XIRF5-miniP-tdTomato-4×ISRE-miniP-mTagBFP2-Blast质粒）大肠杆菌
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Stbl3 (containing pLV3-4XIRF5-miniP-tdTomato-4×ISRE-miniP-mTagBFP2-Blast plasmid) E. coli - Basic Information
Recommended Medium Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C) Growth Conditions 37°C;
18-24h;
AerobicStorage Conditions -80°C Procedure
① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C), shaking rapidly to dissolve;
② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer to a biosafety cabinet for handling;
③ Unscrew the cap, aspirate the entire dissolved solution, and plate onto two corresponding antibiotic-resistant plates, approximately 200μL per plate;
④ Incubate the antibiotic-resistant plates under the above specified conditions for 18-24h before observation (do not seal or wrap the plates);
⑤ Pick a single colony and inoculate into 50mL liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37°C on a shaker;
⑥ According to experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;Sharing Method Public welfare sharing
Partial purchase records(bought amounts latest8)
| Username | Quantity | bought time |
| Ma*** | 1 | 2026-09-05 |
| Be*** | 1 | 2026-08-02 |
| Wi*** | 1 | 2026-07-14 |
| La*** | 1 | 2026-07-04 |
| Pa*** | 1 | 2026-06-30 |
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