DH5α (containing pET-19b plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24 h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (mold at 30℃, bacteria at 37℃), shaking rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol cotton pad for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and plate it onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic-resistant recommended medium, culture overnight at 37℃ on a shaker; ⑥ According to experimental needs, pipette an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public welfare sharing