DH5α (containing pET-3b plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Disinfect the outer surface of the cryovial with an alcohol wipe, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and spread it onto 2 plates with the corresponding antibiotic, approximately 200μL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50mL of liquid LB antibiotic Recommended Medium, then culture overnight at 37°C on a shaker; ⑥ According to experimental needs, take an appropriate amount of the bacterial culture for plasmid extraction.
Sharing Method	Public welfare sharing