DH5α (containing pET-26b plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24 hours; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (molds at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate all the dissolved liquid, and inoculate onto 2 corresponding antibiotic-resistant plates, approximately 200 μL per plate; ④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 hours before observation (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic-resistant recommended medium, and culture overnight at 37°C on a shaker; ⑥ According to experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing