Escherichia coli Stbl3(pLV3-U6-MCS-shRNA-mCherry-Puro)
Stbl3（含pLV3-U6-MCS-shRNA-mCherry-Puro质粒）大肠杆菌
All categories
View History [Clear]
Details
Stbl3 (Containing pLV3-U6-MCS-shRNA-mCherry-Puro Plasmid) E. coli - Basic Information
Recommended Medium Yeast Extract: 5.0 g, Peptone: 10.0 g, NaCl: 10.0 g, pH: 7.0 (25℃) Growth Conditions 37℃;
18-24 h;
AerobicStorage Conditions -80℃ Procedure
① Thaw the cryovial in a water bath (mold 30℃, bacteria 37℃) and shake rapidly to dissolve;
② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer it to a biosafety cabinet for handling;
③ Unscrew the cap, draw out all the dissolved solution, and spread it onto 2 corresponding antibiotic-resistant plates, about 200 μL per plate;
④ Incubate the antibiotic-resistant plates under the specified conditions above for 18-24 h before observation (do not seal or wrap the plates);
⑤ Pick a single colony and inoculate it into 50 mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37℃ on a shaker;
⑥ According to experimental needs, take an appropriate amount of the bacterial culture for plasmid extraction;Sharing Method Public Welfare Sharing
Partial purchase records(bought amounts latest7)
| Username | Quantity | bought time |
| Mi*** | 1 | 2026-09-09 |
| Um*** | 1 | 2026-09-06 |
| Ga*** | 1 | 2026-09-04 |
| Sa*** | 1 | 2026-08-30 |
| Fi*** | 1 | 2026-08-15 |
User Comment(Total0User Comment Num)
- No comment
+86 571 56623320
SUNLONG BIOTECH
