BL21(DE3) (containing pET-32a(+)-ORF7A plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic;
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (37°C) with rapid shaking to dissolve; ② Wipe the exterior of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, draw out all the dissolved liquid, and dispense onto 1-2 agar plates (approximately 200 μL per plate); ④ Spread evenly and culture under the above-mentioned growth conditions.
Reference Application	Extraction of pET-32a(+)-ORF7A plasmid. Gene species: SARS-CoV-2
Sharing Method	Public welfare sharing

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