Escherichia coli Stbl3(pLV3-U6-MCS-sgRNA-Cas9-YPet-Blast)
Stbl3（含pLV3-U6-MCS-sgRNA-Cas9-YPet-Blast质粒）大肠杆菌
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Stbl3 (Containing pLV3-U6-MCS-sgRNA-Cas9-YPet-Blast Plasmid) E. coli - Basic Information
Recommended Medium Yeast Extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃) Growth Conditions 37℃;
18-24h;
AerobicStorage Conditions -80℃ Procedure
① Thaw the cryovial in a water bath (mold at 30℃, bacteria at 37℃) and shake rapidly to dissolve;
② Disinfect the outer wall of the cryovial with an alcohol cotton pad, then transfer to a biosafety cabinet for handling;
③ Unscrew the cap, aspirate the entire dissolved liquid, and inoculate onto 2 corresponding antibiotic plates, approximately 200μL per plate;
④ Incubate the antibiotic plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates);
⑤ Pick single colonies and inoculate into 50mL liquid LB antibiotic Recommended Medium, culture overnight at 37℃ on a shaker;
⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;Sharing Method Public Welfare Sharing
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| Username | Quantity | bought time |
| Ga*** | 1 | 2026-09-14 |
| Ad*** | 1 | 2026-09-05 |
| Be*** | 1 | 2026-08-28 |
| Sa*** | 1 | 2026-07-01 |
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