Stbl3 (Containing pLV3-U6-MCS-sgRNA-Fluc-Blast Plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25℃)
Growth Conditions	37℃; 18-24h; Aerobic
Storage Conditions	-80℃
Procedure	① Thaw the cryovial in a water bath (molds at 30℃, bacteria at 37℃), shaking rapidly to dissolve; ② Wipe the outside of the cryovial with an alcohol swab for disinfection, then transfer to a biosafety cabinet for handling; ③ Unscrew the cap, aspirate the entire dissolved solution, and inoculate onto 2 plates with the corresponding antibiotic, approximately 200μL per plate; ④ Incubate the antibiotic plates under the specified conditions above for 18-24h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate into 50mL liquid LB medium with the corresponding antibiotic, culture overnight at 37℃ on a shaking incubator; ⑥ According to experimental needs, take an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing

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