Stbl3 (containing pLV3-U6-MCS-shRNA-Puro plasmid) E. coli - Basic Information

Recommended Medium	Yeast extract: 5.0g, Peptone: 10.0g, NaCl: 10.0g, pH: 7.0 (25°C)
Growth Conditions	37°C; 18-24h; Aerobic
Storage Conditions	-80°C
Procedure	① Thaw the cryovial in a water bath (mold at 30°C, bacteria at 37°C) and shake rapidly to dissolve; ② Disinfect the outer wall of the cryovial with an alcohol swab, then transfer it to a biosafety cabinet for handling; ③ Unscrew the vial cap, aspirate all the dissolved solution, and spread it onto 2 corresponding antibiotic-resistant plates, approximately 200μL per plate; ④ Incubate the antibiotic-resistant plates under the above-specified conditions for 18-24h, then observe (do not seal or wrap the plates); ⑤ Pick a single colony and inoculate it into 50mL of liquid LB antibiotic-resistant Recommended Medium, culture overnight at 37°C on a shaker; ⑥ According to experimental needs, aspirate an appropriate amount of bacterial culture for plasmid extraction;
Sharing Method	Public Welfare Sharing